![]() ![]() You can use the demo data to compare with your own 10x Genomics Chromium Single Cell Gene Expression runs. Because these are public data sets, these are free and do not count against storage limits. These runs can be found under the “single cell” category. Check our previous blog post on our Demo Data section in BaseSpace™ Sequence Hub for additional details on how to access published run data.īelow are two links to directly import the runs and project folders into your BaseSpace account. To view a set of sequencing runs for 10x Genomics Chromium Single Cell Gene Expression libraries run on both NextSeq™ 2000 P3 flow cells and NovaSeq™ 6000 SP v1.5 flow cells, please visit BaseSpace™ Sequence Hub. The 10x Genomics Chromium Single Cell Gene Expression assay is compatible with all Illumina ® platforms, but we recommend using NextSeq™ 550/1000/2000 and NovaSeq™ 6000 to be able to sequence multiple samples per run (see table 1 below).ġ0x Genomics Chromium Single Cell Gene Expression runs available on BSSH demo data page Single-cell sequencing data can also be analyzed using the Illumina DRAGEN™ Single-Cell app, available in the cloud on BaseSpace™ Sequence Hub, or locally on the NextSeq 1000 and NextSeq 2000 instruments. Sequencing for the single-cell gene expression library then happens on Illumina ® instruments, followed by analysis and data visualization using 10x Genomics Cell Ranger and Loupe Browser software. Today, we are excited to announce that we have published sequencing data for this product on BaseSpace™ Sequence Hub, including one set of libraries sequenced on the NextSeq™ 2000 P2 flow cell, and another set of libraries sequenced on a NovaSeq™ 6000 SP v1.5 flow cell.ġ0x Genomics Chromium Single Cell Gene Expression workflowĪ typical single cell RNA-Seq experiment with 10x Genomics Chromium Single Cell Gene Expression starts with a single cell or single nuclei suspension, followed by library preparation on the Chromium instrument (figure 1 below).įigure 1: The overall workflow for preparing 10x Genomics Single Cell Gene Expression libraries starts with a single cell or single nuclei suspension, followed by microfluidic single cell partitioning and barcoding with the Chromium instrument. The Chromium Single Cell Gene Expression assay provides single cell transcriptome 3' gene expression and multiomic capabilities. 10 x Genomics is a partner that develops multiple single cell and spatial biology solutions. ![]() Single-cell RNA-sequencing is a next-generation sequencing (NGS) method that examines the transcriptomes of individual cells, providing a high-resolution view of cell-to-cell variation. ![]() Today, we’re sharing the fifth in a series of posts that shed more light on new run data publicly available on BaseSpace ™ Sequence Hub (BSSH). ![]()
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